Current Surveillance Programmes

2006 (FIRM funded)
Prevalence and epidemiology of emergent strains of verocytotoxigenic E. coli O157, O26 and O111 in Irish food animals at the pre-harvest and harvest levels of the food chain. Ref: 05/R&D/D/364
2006 (Food Institutional Research Measure funded)
Prevalence and characterisation of Yersinia enterocolitica in animal populations in Ireland.06-RD-D-419
2006 (FIRM funded)
Antimicrobial Resistance in Commensal Organisms in Dairy, Pig and Poultry Production in Ireland as a Resevoir of Resistance for Animal and Zoonotic Pathogens. Ref:
2006 ongoing (Food Institutional Research Measure funded)
Isolation and molecular characterisation of Mycobacterium avium subsp Paratuberculosis in Ireland. Ref: 06RDCIT414
2004 (FSAI funded)
Surveillance of Dairy Herds Supplying raw milk to Farmhouse Cheesemakers for Verocytotoxin producing Escherichia coli 0157:H7 and other VTEC. Research Partners: Faculty of Veterinary Medicine, UCD & Cais
May 2003 (Safefood funded)
Surveillance and Risk Assessment for MAP culture positive herds and suspect positive herds.
Research Partners: Veterinary College, UCD, CVRL DAF, QUB, CCC.
June 2003 (CIT funded)
Detection and Analysis of Selected Pathogens in Water Supplies of Selected Target and Cohort Dairy Herds.
Research Partners: Department of Biological Sciences, CIT, Veterinary Department Cork County Council, Herd Owners.
2001-2004 ongoing (Safefood funded)
'Detection and Molecular Characterisation of Mycobacterium avium subsp. Paratuberculosis (MAP) Salmonella Species and Enterohaemmorhagic E. coli, including verocytotoxin producing E.coli in Unpasteurised Milk, using Milk Sock Filters.'
Research Partners: Cork Institute of Technology, Queen's University, Belfast, Cork County Council, Veterinary Department
1991 (EPA ongoing funded)
Surveillance of Dairy Herds adjoining industrial complexes in County Cork.
Research Partners: Faculty of Veterinary Medicine, UCD, Teagasc, Veterinary Practitioners, Herd Owners, EPA.
Publications

Buckley, J., O'Donovan, J.V., Berry,D.P., O'Mahony, P., O'Farrell, K.J., (2006) Production and Calving Related Events in Irish Dairy Herds in the vicinity of a Chemical Industrial Complex. Veterinary Record 2006. In Press.

Buckley, J., (2005) Verocytotoxin producing E. coli O157 and other VTEC: Surveillance. Food Safety Authority of Ireland, ISSN, 1393-6972 Vol. 7 issue 2.

Murphy, B.P., Murphy, M., Buckley, J.F., Gilroy, D., Rowe, M.T., McCleery, D., Fanning, S., (2005). In-line milk filter analysis: Escherichia coli O157 surveillance of milk production holdings. Int. Journal of Hygiene and Environmental Health. (In Press)

Buckley, J., Wall. P., (2003) Using animals to monitor the environment and the food chain in the vicinity of incinerators. FSAI Newsletter, Vol. 5, Issue 2.

Hegarty, H., O'Sullivan, M.B., Buckley, J., Foley-Nolan, C., (2002) Unpasteurised Milk Consumption by Farming Families: A Qualitative Study of the Persistence of the Practice. Communicable Disease and Public Health Journal.

Buckley, J., Wall.P., (2002) Levels of Dioxins, Furans and PCB's in Irish Milk 1991 - 2002 FSAI Newsletter, Vol 4, Issue 4.

McKee, R., Grant I.R., Rowe, M.T., Buckley, H.G., Buckley, J. F., Fanning. S., (2002) Examination of in-line milk filters to detect mycobacterium avium subsp. Paratuberculosis infection at farm level. Presented at the 7th International Colloquium on Paratuberculosis in Bilbao, Spain, from 10-14th June 2002.

Murphy, B.P., Fanning, S., Buckley, H.G., Buckley, J.F., McKee. R.,(2002) Detection of Pathogenic Microorganisms in Raw Milk and Indicator Microorganisms in Water Samples from Dairy Farms in the Cork Region. Presented at the 32nd Food Science and Technology Conference, UCC, 11-12th September 2002.

O'Sullivan, M., Buckley, J., Cryan. B. (2002) Human Brucellosis - Taking Stock. Published in the Irish Veterinary Journal Vol. 55(1) Jan.

Fanning, S., Daly, M., O'Halloran, F., Buckley, G., Buckley, J., Power. E., (2002) Applications of DNA-based analytical methods. Part two: DNA diagnostics.
Irish Veterinary Journal Vol. 55(5) May.

Murphy, B.P., Fanning, S., Buckley, H.G., Buckley, J.F., McKee, R., McCleery, D.R., Rowe, M.T., Gilory. D., (In press) (2002) Prevalence of Salmonella spp. detected from in-line milk filters, procured from Dairy Production Holdings in the Munster region of Ireland.

Murphy, B.P., Fanning, S., Buckley, H.G., Buckley, J. F., Mckee. R., (In Press) (2002) Incidence of Verocytotoxigenic Escherichia coli determined following milk filter analysis from a subset of Liquid Milk Production Holdings in the southern region of Ireland, and microbiological assessment of the water from these holdings.

Crilly, J., Power, E., Fanning, S., Cryan, B., Buckley, J. (2001) Eqidemiology of Salmonella infection in the South of Ireland. Irish Journal of Agricultural and Food Research. 40, 215-226.

Crilly, J., Power, E., Fanning, S., Cryan, B., Buckley, J. (2001) Epidemiology of Salmonella infection in the South of Ireland. Irish Journal of Agricultural and Food Research. 40, 215-226.

Buckley, J.F., Buckley, G.N., (2000) A report on the isolation of verotoxinogenic Escherichia coli from unpasteurised milk - the public health implications. Irish Veterinary Journal. Volume 53(2): February, 79-80.

Buckley, J., McRory, F., O'Mahony, P. (2000) On Farm Study of Consumption of Unpasteurised Milk Published FSAI Research Reports.

Daly, M., Buckley, J., Power, E., O'Hare, C., Cormican, M., Cryan, B., Wall, P.G., Fanning, S., (2000) Appl. Molecular Characterization of Irish Salmonella enterica Serotype Typhimurium: Detection of Class I Integrons and Assessment of Genetic Relationships by DNA Amplification Fingerprinting. Env. Microbiol, 66: 615-619.

Fanning, S., Daly, M., Lucey, B., Buckley, J., Power, E., Cryan, B., Irish. J., (2000) Molecular characterisation and chromosomal mapping of antimicrobial resistance gene cassettes in Irish Salmonella enterica seotype Typhimurium: Comparison with Campylobacter spp. Agri. Food Res. 39: 283-293.

Buckley, J., Larkin.H., (1998) Health Surveillance of cattle in the vicinity of a chemical industrial complex. The Veterinary Record. Sept. 19th, Vol.143, 323-326.

Foley-Nolan, C., Buckley, J., O'Sullivan, E., Cryan. B., (1998) United front - veterinary and medical collaboration. Irish Medical Journal, May/June Vol 91. Number 3, 95-96.

POSTERS PRESENTATIONS

MLVA characterization of Irish Verocytotoxigenic Escherichia coli O157 from milk production holdings

Multiple-locus variable number of tandem repeat analysis (MLVA) of Escherichia coli O157 isolates.

Surveillance of dairy herds supplying raw milk to farmhouse cheese makers for E. coli O157, O26 and O111.

Prevalence of Emerging Pathogens in Irish Dairy Herds.

Prevalence of Verocytotoxigenic Eacherichia coli O157:H7 and Salmonella spacios in Milk Filters from Irish Dairy Herds.